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Technical Support

Phycobiliprotein
1. Is it normal that precipitate forms during conjugation ?

No, it is caused by an over conjugated condition.

2. What is the pH tolerance range of Phycobiliproteins?

The normal pH tolerance is 6.5~8.5. The quality of the product will be damaged if pH value is outside of this range.

3. Under proper storage conditions, how long could phycobiliprotein be stored?

With the packaging supplied by Flogen and storage at 2~8℃ away from light, the shelf life is 3 years.

4. What is the heat tolerance range of Phycobiliprotein?

When the temperature is below 37℃ with the package and solution supplied by Flogen, the quality of phycobiliprotein product can be highly stable or even unchanged for several days. However, when the temperature reaches above 37℃, phycobiliprotein will denature easily within a short period of time.

Lyophilized Phycobiliprotein
1. Comparing with the Phycobiliprotein products in ammonium sulfate, what are the advantages of lyophilized phycobiliproteins?

l.Ready-to-use:It is ready to use without any pre-treatment steps, such as buffer exchange, concentration adjustment, quality checks, etc. In addition, because lyophilization process does not include any material that affects conjugation, there’s no need to worry about the quality of conjugates which, on the other hand, will be affected if there is an incomplete elimination of ammonium sulfate in the ammonium sulfate solution form. 2. Stability:Higher stability, longer shelf life, and also much easier to transport. 3. Flexible production capacity:Available in small and large quantity production 4. Quality:The quality of lyophilized phycobiliproteins is the same as phycobiliproteins in saturated ammonium sulfate solution; their purity and fluorescence performance are the same. 5. Economical effectiveness: Decrease cost in packing and transportation due to much smaller size of the product; reduced labor cost, time consumed, and sample wasted during protein purification in ammonium sulfate solution. 6. Reproducibility: Reducing deviation between experiments, increasing batch-to-batch consistency.

2. Does lyophilization change the property of phycobiliproteins?

Our R&D team has gone through numerous experiments and validations, and has developed the lyophilization process without altering the fluorescent intensity and light absorbance property of phycobiliproteins. Moreover, Lyo Flogen® has passed strict quality tests to ensure it possesses the quality of phycobiliproteins stored in saturated ammonium sulfate solution.

3. Does the lyophilized phycobiliprotein follow the same conjugation procedures as the Phycobiliprotein products store in ammonium sulfate solution?

Yes, please follow your conjugation protocols but skip the pretreatment procedures such as dialysis or desalting column to remove ammonium sulfate. Lyophilized form can save you a lot of time in the pre-treatment step and it can help avoid any change on purity or quality, which tend to affect your experiment later on.

4. How long can the product be stored without altering its properties when it is reconstituted in the buffer?

Because there are no preservatives in lyophilized phycobiliprotein and the package is not sterilized, to preserve our product from other microorganisms or enzymes, or to maintain its original property, we do not recommend long term storage of re-suspended lyophilized phycobiliprotein or freezing and thawing upon usage. We suggest you to re-suspend it in concentration greater than 10mg/ml, stored it in 2~8℃and use the product within a week.

5. Are there any salts in lyophilized phycobiliproteins’ formula that will affect conjugation process?

No ammonium sulfate or other material that will interfere with the conjugation process is added in this product.

Recombinant Protein
1. What kind of buffer should we use to reconstitute the recombinant protein product?

Please follow the instruction to reconstitute the products. If the product is sterile, please follow the operation procedure for sterile product. If not specifically prohibited or the system is allowed, you may add 0.09% NaN3 to inhibit bacterial growth for longer shelf life.

2. How to store and reconstitute the recombinant protein products?

Storage and method of reconstitution vary for different products. Please follow the instruction in the package to store the lyophilized product in 2~8 ℃ or -20 ℃. Please follow the instruction to reconstitute the products. If you are unable use all the product at once, we recommend you to aliquot the product into smaller portions for each single usage and store them in -80℃ to avoid freeze and thaw.

3. Is the product sterilized?

If it states “sterilized” in the instruction, we recommend you to store and operate under sterilized conditions.

4. How long is the shelf life of the product?

The shelf life varies. Please refer to the label or instruction in you package. We can guarantee the valid period of our recombinant protein products is at least one year from the date you receive the product.